- Command Line interface for Linux and Windows
- Single Directory Upload
- MiSeq/MiniSeq/NextSeq Sequencing Run Parser
- Directory Parsing
- Batch Uploads
- Can be used with cron/windows task scheduler for automated uploads
- File upload checksum validation
- Post-processing tasks
- Pause and resume uploads
You can find a tutorial and walkthrough on the phac-nml github https://github.com/phac-nml/irida-uploader-tutorial
Download / Install / Setup
The IRIDA Uploader can be run on any operating system that supports Python.
You can download the source code on GitHub.
You can download pre-built packages for Windows from our GitHub releases page.
Run an installer (links above) and follow along with the install wizard.
You will need to configure your uploader before running. See Configuration for details
Make sure Python 3.5 or newer is installed
$ python3 --version
If python3 is not installed, install with
$ sudo apt-get install python3
$ sudo apt-get install python3-pip
Install virtualenv and setuptools
$ pip install virtualenv $ pip install setuptools
If you already have these packages installed, ensure they are up to date
$ pip install virtualenv -U $ pip install setuptools -U
Download the source code
$ git clone https://github.com/phac-nml/irida-uploader $ cd irida-uploader
Build a virtualenv and install the dependencies automatically with
You will need to configure your uploader before running.
You will need to configure IRIDA and the uploader to upload files.
If you do not create a configuration file, IRIDA uploader will create one for you with default values the first time it try's to upload.
You will need to edit this file with your IRIDA credentials, and the parser that matches your data.
Choose a Parser
The config file has a
parser field that you can use to parse different directory structures
We currently support the following:
directory : Generic Directory
miseq_v26 : Miseq (Control Software Version 2.6)
miseq_v31 : Miseq (Control Software Version 3.1)
miniseq : MiniSeq
iseq : iSeq
nextseq : NextSeq
Starting an upload
You can upload with the following commands
Open a Command Prompt terminal and use the
iridauploader command to upload
C:\Users\username> iridauploader \path\to\my\samples\
irida-uploader.sh script included with the source code to upload.
After uploading, an
irida_uploader_status.info file will be created which indicates if a run is complete, or has failed
You can delete this file to make it ready for reupload, or use the
--force option when running the uploader to ignore the status of a run directory.
You can upload a directory containing run directories by using the
--batch option when running the uploader.
Example batch upload directory:
./irida-uploader.sh --batch /path/to/BatchDirectoryToUpload/
. └── BatchDirectoryToUpload ├── Run_1 │ ├── SampleSheet.csv │ └── <other run files> ├── Run_2 │ ├── SampleSheet.csv │ └── <other run files> └── Run_3 ├── SampleSheet.csv └── <other run files>
--force option can be used with the
WARNING! When uploading
nextseq data and using
--batch upload with an auto-upload script, incomplete fastq files could be uploaded if
bcl2fastq has not finished when the upload begins.
Logs about individual runs are written to the sequencing run directory that they are uploaded from.
Full debug logs are written to your system default logging directory
Check the Errors Page for help with common errors.
Found a bug or have an idea for a feature?
Create an issue on our GitHub Issues Board
Want to create a parser for a sequencer that we don't yet support or have an idea for an IRIDA related project?