The Software on the NextSeq2000 does not allow for automatic generation of a SampleSheet that includes a project column, so create a copy of the sample sheet called UploadList.csv and add the
Sample_Project column ourselves.
If you would like to use this parser you will also need to create this new file and
Please verify your file structure is correct.
. ├── CopyComplete.txt ├── UploadList.csv ├── Analysis │ └── 1 │ └── Data │ └── fastq │ ├── sample1_S1_L001_R1_001.fastq.gz │ ├── sample1_S1_L001_R2_001.fastq.gz │ ├── sample2_S1_L001_R1_001.fastq.gz │ ├── sample2_S1_L001_R2_001.fastq.gz │ ├── sample3_S1_L001_R1_001.fastq.gz │ └── sample3_S1_L001_R2_001.fastq.gz
Example files can be found:
Preparing your nextseq2000 upload list
Before using the uploader, you must prepare your sequencing run with IRIDA-specific project IDs.
Copy and rename the CSV file made by the software to
UploadList.csv (It will have a name something like
Then add the
Sample_Project column to the
An example completed
UploadList.csv with the
Sample_Project column filled in looks like:
[Header] FileFormatVersion,2 Run Name,NGS1001 InstrumentPlatform,NextSeq1k2k InstrumentType,NextSeq2000 [Reads] Read1Cycles,101 Read2Cycles,101 Index1Cycles,8 Index2Cycles,8 [BCLConvert_Settings] SoftwareVersion,3.5.8 AdapterRead1,CTGTCTTCTCTCTC AdapterRead2,CGTCTCTCTCTCTC [BCLConvert_Data] Sample_ID,Index,Index2,Sample_Project sample1,ATCGAAA,ATCGAAA,1 sample2,ATCGAAA,ATCGAAA,1 sample3,ATCGAAA,ATCGAAA,2